List of publications
Publications (Compact list)
| Date & Citations | Publication | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Original Research Article | 2023-09 | Nile red-based lipid fluorometry protocol and its use for statistical optimization of lipids in oleaginous yeasts | Applied Microbiology and Biotechnology | 10.1007/s00253-023-12786-9 | 107 | 7313-7330 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s00253-023-12786-9-citation.ris | B. Ouellet; Z. Morneau; A. M. Abdel-Mawgoud | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-47a8c2e219915f821b7c74770dfeb206-ff-s00253-023-12786-9-5.pdf | |
| Original Research Article | 2023-10 | Strong expression of Cas9 under a new 3′-truncated TEF1α promoter enhances genome editing in Yarrowia lipolytica | Current Research in Biotechnology | 10.1016/j.crbiot.2023.100147 | 6 | 100147 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S2590262823000291.ris | B. Ouellet; A. M. Abdel-Mawgoud | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-5e789f507c2284b283b766e2bd5dfe0c-ff-1-s2.0-S2590262823000291-main.pdf | |
| Original Research Article | 2022-02 | Identification of putative producers of rhamnolipids/glycolipids and their transporters using genome mining | Current Research in Biotechnology | 10.1016/j.crbiot.2022.02.002 | 4 | 152-166 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S2590262822000077.ris | M. Magri; A. M. Abdel-Mawgoud | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-c8fd4173478eb550bfe8f97a3a55184e-ff-1-s2.0-S2590262822000077-main.pdf | |
| Original Research Article | 2020-08 | Improving CRISPR/Cas9-mediated genome editing efficiency in Yarrowia lipolytica using direct tRNA-sgRNA fusions | Metabolic Engineering | 10.1016/j.ymben.2020.07.008 | 62 | 106-115 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s00253-023-12786-9-citation.ris | A. M. Abdel-Mawgoud; G. Stephanopoulos | ||
| Review Article | 2018-11 | Metabolic engineering in the host Yarrowia lipolytica | Metabolic Engineering | 10.1016/j.ymben.2018.07.016 | 50 | 192-208 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S1096717618302738.ris | A. M. Abdel-Mawgoud; K. A. Markham; C. M. Palmer; N. Liu; G. Stephanopoulos; H. S. Alper | ||
| Review Article | 2018-03 | Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering | Synthetic and Systems Biotechnology | 10.1016/j.synbio.2017.12.001 | 3 | 1 | 45370 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S1074552113004195.ris | A. M. Abdel-Mawgoud; G. Stephanopoulos | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-83cd398f6ea755253e6dbe4ef1fca018-ff-1-s2.0-S2405805X17300972-main.pdf |
| Original Research Article | 2014-01 | A Stereospecific Pathway Diverts β-Oxidation Intermediates to the Biosynthesis of Rhamnolipid Biosurfactants | Chemistry & Biology (Now: Cell Chemical Biology) | 10.1016/j.chembiol.2013.11.010 | 21 | 1 | 156-164 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S1074552113004195.ris | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-4b11699804aef7f62072232804804eac-ff-1-s2.0-S1074552113004195-main.pdf |
| Original Research Article | 2013-09 | A chiral high-performance liquid chromatography–tandem mass spectrometry method for the stereospecific analysis of enoyl-coenzyme A hydratases/isomerases | Journal of Chromatography A | 10.1016/j.chroma.2013.07.049 | 1306 | 37-43 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S0021967313010935.ris | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-9d867fc38cb89be5a90f724b40ed6ab7-ff-Abdel-Mawgoud-2013-A-chiral-high-perfor.pdf | |
| Original Research Article | 2013-03 | Comparative Analysis of Rhamnolipids from Novel Environmental Isolates of Pseudomonas aeruginosa | Journal of Surfactants and Detergents | 10.1007/s11743-013-1462-4 | 16 | 673-682 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s11743-013-1462-4-citation.ris | M. G. Rikalovic; A. M. Abdel-Mawgoud; E. Déziel, G. Dj. Gojgic-Cvijovic; Z. Nestorovic; M. M. Vrvic; I. M. Karadzic | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-85b79e86637de2b4e329ce3c3431f120-ff-s11743-013-1462-4.pdf | |
| Review Article | 2010-03 | Rhamnolipids: diversity of structures, microbial origins and roles | Applied Microbiology and Biotechnology | 10.1007/s00253-010-2498-2 | 86 | 1323–1336 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s00253-010-2498-2-citation.ris | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-4f351982a2f8aae68d832bbbba1d534d-ff-s00253-010-2498-2-1.pdf | |
| Original Research Article | 2008-06 | Characterization of Rhamnolipid Produced by Pseudomonas aeruginosa Isolate Bs20 | Applied Biochemistry and Biotechnology | 10.1007/s12010-008-8285-1 | 157 | 329-345 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s12010-008-8285-1-citation.ris | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-621776cfa9e2d27b53fa65c8858517ab-ff-s12010-008-8285-1.pdf | |
| Original Research Article | 2008-04 | Characterization of Surfactin Produced by Bacillus subtilis Isolate BS5 | Applied Biochemistry and Biotechnology | 10.1007/s12010-008-8153-z | 150 | 3 | 289-303 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s12010-008-8153-z-citation.ris | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-49a371e3ad8e70901e5197684e9f3a6a-ff-s12010-008-8153-z.pdf |
| Original Research Article | 2008-02 | Optimization of Surfactin Production by Bacillus subtilis Isolate BS5 | Applied Biochemistry and Biotechnology | 10.1007/s12010-008-8155-x | 150 | 305-325 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_s12010-008-8155-x-citation.ris | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-d3a63bfe9b746472f827ea1533fd0be8-ff-s12010-008-8155-x.pdf | |
| Original Research Article | 2007-10 | Microbial production of surfactants: Screening and identification of two promising isolates and their biosurfactants | Egyptian Journal of Biotechnology | 27 | 166-185 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/citation-screening-of-producers.ris | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-f3e1ed4235a62e095faae9b926d88783-ff-ScreeningBiosurfactant.pdf | ||
| Book Chapter | 2014-03 | Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids | Pseudomonas Methods and Protocols. Methods in Molecular Biology | 10.1007/978-1-4939-0473-0_30 | 1149 | 359–373 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_978-1-4939-0473-0_30-citation.ris | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-51222d686dde946b1795bdf120e7145b-ff-2014ee-Abdel-Mawgoud-Pseudomonas_Methods-and-P.pdf | |
| Book Chapter | 2010-09 | Rhamnolipids: Detection, Analysis, Biosynthesis, Genetic Regulation, and Bioengineering of Production | Biosurfactants. Microbiology Monographs | 10.1007/978-3-642-14490-5_2 | 20 | 13–55 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/10.1007_978-3-642-14490-5_2-citation.ris | A. M. Abdel-Mawgoud; R. Hausmann; F. Lépine; M. M. Müller; E. Déziel | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-1606a0b92864c94baafc52e95cc4d5ae-ff-Book-Chapter.pdf | |
| Patent | 2015-06 | Modulation of the beta-oxidation pathway in the control of rhamnolipid production | World Intellectual Property Organization - PCT | 1-83 | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-786903729285bd23542251a8d50c283a-ff-Patent-combined_opt.pdf | ||||
| Original Research Article | 2024-02 | Production of genetically engineeered designer biodiesel from yeast lipids | Current Research in Biotechnology | 10.1016/j.crbiot.2024.100189 | 7 | 100189 | https://abdel-mawgoud.com/wp-content/uploads/fluentform/S2590262824000157.ris | B. Ouellet; A. M. Abdel-Mawgoud | https://abdel-mawgoud.com/wp-content/uploads/fluentform/1-s2.0-S2590262824000157-main.pdf |
Publications (Graphical list)
| Publication & Citations | Date, Publisher & Statistics | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Nile red-based lipid fluorometry protocol and its use for statistical optimization of lipids in oleaginous yeasts
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Original Research Article | 2023-09 | 10.1007/s00253-023-12786-9 | Applied Microbiology and Biotechnology | 107 | 7313-7330 | Citation file | B. Ouellet; Z. Morneau; A. M. Abdel-Mawgoud | As lipogenic yeasts are becoming increasingly harnessed as biofactories of oleochemicals, the availability of efficient protocols for the determination and optimization of lipid titers in these organisms is necessary. In this study, we optimized a quick, reliable, and high-throughput Nile red-based lipid fluorometry protocol adapted for oleaginous yeasts and validated it using different approaches, the most important of which is using gas chromatography coupled to flame ionization detection and mass spectrometry. This protocol was applied in the optimization of the concentrations of ammonium chloride and glycerol for attaining highest lipid titers in Rhodotorula toruloides NRRL Y-6987 and Yarrowia lipolytica W29 using response surface central composite design (CCD). Results of this optimization showed that the optimal concentration of ammonium chloride and glycerol is 4 and 123 g/L achieving a C/N ratio of 57 for R. toruloides, whereas for Y. lipolytica, concentrations are 4 and 139 g/L with a C/N ratio of 61 for Y. lipolytica. Outside the C/N of 33 to 74 and 45 to 75, respectively, for R. toruloides and Y. lipolytica, lipid productions decrease by more than 10%. The developed regression models and response surface plots show the importance of the careful selection of C/N ratio to attain maximal lipid production. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-672263a211a1f1e2c7919b1c658c7271-ff-lipogenesis.png | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-ff7fab06ddc09e8ca1791bf8cdcdd754-ff-NR-optimzation.png | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-407f2810bf5cb048dd3ba98ebe45083a-ff-CDC.png | <a href="https://www.scimagojr.com/journalsearch.php?q=14957&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=14957" alt="SCImago Journal & Country Rank" /></a> | ||
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Strong expression of Cas9 under a new 3′-truncated TEF1α promoter enhances genome editing in Yarrowia lipolytica
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Original Research Article | 2023-10 | 10.1016/j.crbiot.2023.100147 | Current Research in Biotechnology | 6 | 100147 | Citation file | B. Ouellet; A. M. Abdel-Mawgoud | The non-conventional yeast Yarrowia lipolytica is gaining interest in biotechnology as a workhorse for the production of proteins, lipids and other biomolecules. Site-specific genome editing is however limited in this yeast. Although, this was much improved by the recent adaptation of a CRISPR-Cas9 genome editing protocol for Y. lipolytica based on a tRNA-sgRNA fusion, yet, in the latter protocol, Cas9 is under the control of a synthetic hybrid promoter, pUAS1B8-TEF(136) that is associated with some drawbacks. This hybrid promoter contains tandem repeats are suggested to cause in vivo and in vitro inconveniences like polymerase slippage, random genetic rearrangements and cloning difficulties. Here we report a newly designed synthetic TEF promoter to drive Cas9 expression, pTEF(-41–406)-Kozak, which is a rationally 3′-truncated version of the already known 5′-truncated pTEF(406) promoter of Y. lipolytica fused to a synthetic Kozak sequence. Our comparison of the promoters’ strength using hrGFP reporters and RT-qPCR showed that the synthetic pTEF(-41–406)-Kozak has an equivalent expression strength to that of pTEF(406), yet is at least 5 times stronger than the hybrid pUAS1B8-TEF(136). The pTEF(-41–406) promoter mediated high expression of Cas9 and was not associated with any growth defects. Moreover, expression of Cas9 under pTEF(-41–406)-Kozak increased the gene integration efficiency by up to 40 % relative to that when Cas9 is expressed under pUAS1B8-TEF(136). Both pTEF(-41–406) and pUAS1B8-TEF(136) performed equally well as drivers of Cas9 expression with respect to gene deletion as demonstrated both on the genotypic and phenoypic levels. This is the first study conducting rational 3′-truncation in TEF promoter in Y. lipolytica based on in silico analysis of promoter sequence and structure. This approach of promoter engineering can be extended to the engineering of other yeast promoters to generate small-sized synthetic biology parts for convenient engineering of biological systems. This work provides a strong Cas9 expression cassette for more convenient and efficient CRISPR-Cas9-mediated genome editing in Y. lipolytica which will facilitate harnessing the full potential of this industrial strain. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-3c14da80bd346a8c974249038b88f654-ff-CRISPR-Fig1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-0763c6676586b9746c75cef4b2b3a40f-ff-CRISPR-Fig2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-ee9a9934c0fd9b52d228bb01e1b2a666-ff-CRISPR-Fig3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=21101039902&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=21101039902" alt="SCImago Journal & Country Rank" /></a> | ||
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Identification of putative producers of rhamnolipids/glycolipids and their transporters using genome mining
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Original Research Article | 2022-02 | 10.1016/j.crbiot.2022.02.002 | Current Research in Biotechnology | 4 | 152-166 | Citation file | M. Magri; A. M. Abdel-Mawgoud | Rhamnolipids (RLs) are microbial glycolipids (GLs) with interesting structure-dependent bioactivities and physicochemical properties making them suitable for diverse medical and industrial applications. The discovery of RLs with more interesting bioactivities and properties has relied on laborious screening of new RL producers isolated from the environment, and has resulted in the redundant identification of already known RL producers and structures. Here, we present a genome mining approach that enabled the identification of 80 RL-producing species (including the two reference species), 71 of which were previously unreported. Distance trees of two of their RL biosynthetic enzymes, RhlAB, allowed for the identification of 11 distinct clades. Preliminary experimental validation with thin layer chromatography on one non-pathogenic RL/GL producer, Nevskia soli, confirmed its putative production of RLs. Additionally, this study led to the discovery of the putative RL transport mechanism involving three transmembrane proteins whose coding genes are highly conserved and clustered with one of the RL biosynthetic gene clusters in most RL/GL producers identified in this study. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-d2eeae187ae2301bce225d330af3889e-ff-Figure1-1-s2.0-S2590262822000077-ga1_lrg.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-c5d9a7f5f4d040bdb394a5747222ac65-ff-Figure2-1-s2.0-S2590262822000077-gr1_lrg.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-6c809b953bf3ed847847e7e8a6efca19-ff-Figure3-1-s2.0-S2590262822000077-gr5.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=21101039902&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=21101039902" alt="SCImago Journal & Country Rank" /></a> | ||
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Improving CRISPR/Cas9-mediated genome editing efficiency in Yarrowia lipolytica using direct tRNA-sgRNA fusions
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Original Research Article | 2020-08 | 10.1016/j.ymben.2020.07.008 | Metabolic Engineering | 62 | 106-115 | Citation file | A. M. Abdel-Mawgoud; G. Stephanopoulos | Yarrowia lipolytica is an important oleaginous yeast currently used in the production of specialty chemicals and has a great potential for further applications in lipid biotechnology. Harnessing the full potential of Y. lipolytica is, however, limited by its inherent recalcitrance to genetic manipulation. In contrast to Saccharomyces cerevisiae, Y. lipolytica is poor in homology-mediated DNA repair and thus in homologous recombination, which limits site-specific gene editing in this yeast. Recently developed CRISPR/Cas9-based methods using tRNA-sgRNA fusions succeeded in editing some genomic loci in Y. lipolytica. Nonetheless, the majority of other tested loci either failed editing or editing was achieved but at very low efficiency using these methods. Using tools of secondary RNA structure prediction, we were able to improve the design of the tRNA-sgRNA fusions used for the expression of single guide RNA (sgRNA) in such methods. This resulted in high efficiency CRISPR/cas9 gene editing at chromosomal loci that failed gene editing or were edited at very low efficiencies with previous methods. In addition, we characterized the gene editing performance of our newly designed tRNA-sgRNA fusions for both chromosomal gene integration and deletion. As such, this study presents an efficient CRISPR/Cas9-mediated gene-editing tool for efficient genetic engineering of Yarrowia lipolytica. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-5f58d4d6a74a6aa8a4993d4cd3d6213e-ff-Figure-1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-be8761d3d519ba8e9ed8f52f2d453f2f-ff-Figure-2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-be19953c5fe47237c605a0daa5b1166a-ff-Figure-7.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=16339&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=16339" alt="SCImago Journal & Country Rank" /></a> | ||
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Metabolic engineering in the host Yarrowia lipolytica
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Review Article | 2018-11 | 10.1016/j.ymben.2018.07.016 | Metabolic Engineering | 50 | 192-208 | Citation file | A. M. Abdel-Mawgoud; K. A. Markham; C. M. Palmer; N. Liu; G. Stephanopoulos; H. S. Alper | The nonconventional, oleaginous yeast, Yarrowia lipolytica is rapidly emerging as a valuable host for the production of a variety of both lipid and nonlipid chemical products. While the unique genetics of this organism pose some challenges, many new metabolic engineering tools have emerged to facilitate improved genetic manipulation in this host. This review establishes a case for Y. lipolytica as a premier metabolic engineering host based on innate metabolic capacity, emerging synthetic tools, and engineering examples. The metabolism underlying the lipid accumulation phenotype of this yeast as well as high flux through acyl-CoA precursors and the TCA cycle provide a favorable metabolic environment for expression of relevant heterologous pathways. These properties allow Y. lipolytica to be successfully engineered for the production of both native and nonnative lipid, organic acid, sugar and acetyl-CoA derived products. Finally, this host has unique metabolic pathways enabling growth on a wide range of carbon sources, including waste products. The expansion of carbon sources, together with the improvement of tools as highlighted here, have allowed this nonconventional organism to act as a cellular factory for valuable chemicals and fuels. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-7c78cf875e8187aaeb29ae417f2d4b8f-ff-Figure1.png | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-678ab0c0a15c2fcdb715ed66b94763f5-ff-Figure2.png | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-948c923ee5c7a34793e69c72e5bad091-ff-Figure3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=16339&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=16339" alt="SCImago Journal & Country Rank" /></a> | ||
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Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering
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Review Article | 2018-03 | 10.1016/j.synbio.2017.12.001 | Synthetic and Systems Biotechnology | 3 | 1 | 45370 | Citation file | A. M. Abdel-Mawgoud; G. Stephanopoulos | Glycosylated lipids (GLs) are added-value lipid derivatives of great potential. Besides their interesting surface activities that qualify many of them to act as excellent ecological detergents, they have diverse biological activities with promising biomedical and cosmeceutical applications. Glycolipids, especially those of microbial origin, have interesting antimicrobial, anticancer, antiparasitic as well as immunomodulatory activities. Nonetheless, GLs are hardly accessing the market because of their high cost of production. We believe that experience of metabolic engineering (ME) of microbial lipids for biofuel production can now be harnessed towards a successful synthesis of microbial GLs for biomedical and other applications. This review presents chemical groups of bacterial and fungal GLs, their biological activities, their general biosynthetic pathways and an insight on ME strategies for their production. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-9755b120283f44cb8daf6b2be5bd18ec-ff-1-s2.0-S2405805X17300972-gr3_lrg.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-91ad05b6c56b51fccadf01d61e3e5888-ff-1-s2.0-S2405805X17300972-gr4_lrg.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-df85307fdc4bbb70f2c5e8a45d718e09-ff-1-s2.0-S2405805X17300972-gr5.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=21100832688&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=21100832688" alt="SCImago Journal & Country Rank" /></a> | |
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A Stereospecific Pathway Diverts β-Oxidation Intermediates to the Biosynthesis of Rhamnolipid Biosurfactants
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Original Research Article | 2014-01 | 10.1016/j.chembiol.2013.11.010 | Chemistry & Biology (Now: Cell Chemical Biology) | 21 | 1 | 156-164 | Citation file | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | Rhamnolipids are multipurpose surface-active molecules produced by the bacterium Pseudomonas aeruginosa from L-rhamnose and R-3-hydroxyalkanoate (C10±2) precursors. R-3-hydroxyalkanoate precursor is believed to be synthesized de novo. We demonstrate, however, that β-oxidation is the predominant source of this precursor. Inhibition of β-oxidation sharply decreases rhamnolipids production, even when using a nonfatty acid carbon source (glycerol). Isotope tracing shows that β-oxidation intermediates are direct precursors of rhamnolipids. A mutant-based survey revealed an operon coding for enoyl-CoA hydratases/isomerases (ECH/I), named RhlYZ, implicated in rhamnolipids production via an axial role in 3-hydroxyalkanoate synthesis. In vitro, RhlZ is an R-ECH/I transforming 2-decenoyl-CoA, a β-oxidation intermediate, into R-3-hydroxydecanoyl-CoA, the potential rhamnolipids precursor. Interestingly, polyhydroxyalkanoates share with rhamnolipids the RhlYZ-generated R-3-hydroxyalkanoates pool, as demonstrated by the decrease of polyhydroxyalkanoates upon mutation of rhlYZ and the increase of rhamnolipids in a polyhydroxyalkanoates-defective mutant. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-7c9bfd6bd0507ed2d086f765795ce888-ff-1-s2.0-S1074552113004195-gr2_figure1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-5c9aea2bdcda49eeea653a946c6e8391-ff-1-s2.0-S1074552113004195-gr4_lrgFigure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-1091908230f4b5952fd745f438ada222-ff-1-s2.0-S1074552113004195-gr7-Figure-3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=21100456838&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=21100456838" alt="SCImago Journal & Country Rank" /></a> | |
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A chiral high-performance liquid chromatography–tandem mass spectrometry method for the stereospecific analysis of enoyl-coenzyme A hydratases/isomerases
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Original Research Article | 2013-09 | 10.1016/j.chroma.2013.07.049 | Journal of Chromatography A | 1306 | 37-43 | Citation file | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | The enzymes catalyzing the stereospecific hydration of 2-enoyl-CoA into the corresponding S- or R-3-hydroxyacyl-CoA are named enoyl-CoA hydratases (ECH), where the S-specific is called ECH-1 and the R-specific is called ECH-2. Current ECH assays are mostly based on spectrophotometric methods. Amongst many limitations, these methods do not directly measure the 3-hydroxyacyl-CoA produced, neither do they allow determination of its stereospecific configuration. We have developed a chiral HPLC method coupled with tandem mass spectrometry (MS) for the sensitive, direct, stereospecific and quantitative analysis of ECH-1/-2 reaction products, or R-/S-3-hydroxyalkanoates in general. The method is based on the reaction of the 3-hydroxyl group on the chiral carbon with 3,5-dimethylphenyl isocyanate, creating a urethane derivative which is then chirally resolved on a chiral HPLC column having 3,5-dimethylphenyl carbamate-derivatized cellulose as the chiral stationary phase. The resolved urethane derivatives are detected using tandem MS in the multiple reactions monitoring (MRM) negative electrospray ionization mode by monitoring the free hydroxy fatty acid fragment ion liberated from its parent urethane derivative. The method resolves the R-/S-enantiomers of 3-hydroxy fatty acid homologues ranging from C6 to C16. Using this method, the net ECH activity present in clarified cell lysates of the bacterium Pseudomonas aeruginosa cultivated in a rich medium was found to be of both ECH-1 and ECH-2. Interestingly, the clarified cell lysate of Escherichia coli cultivated also in a rich medium displayed mainly an ECH-1 (S-specific) activity. This method will facilitate the quantification and stereospecific characterization of ECHs, as well as the chiral lipid profiling of bacterial secondary metabolites containing hydroxyalkanoic acid moieties. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-2cd1d46520966d995340cd00153b0489-ff-Figure-1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-33da121964d41b1b50d372e99ad36880-ff-Figure-2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-f7357dc9cae6ea4e42df38aed76c36fd-ff-Figure-3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=130000&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=130000" alt="SCImago Journal & Country Rank" /></a> | ||
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Comparative Analysis of Rhamnolipids from Novel Environmental Isolates of Pseudomonas aeruginosa
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Original Research Article | 2013-03 | 10.1007/s11743-013-1462-4 | Journal of Surfactants and Detergents | 16 | 673-682 | Citation file | M. G. Rikalovic; A. M. Abdel-Mawgoud; E. Déziel, G. Dj. Gojgic-Cvijovic; Z. Nestorovic; M. M. Vrvic; I. M. Karadzic | A comparative analysis of rhamnolipids from environmental isolates of Pseudomonas aeruginosa was undertaken to evaluate strain-specific rhamnolipid fingerprints obtained under different growth conditions. Environmental isolates of P. aeruginosa produced rhamnolipids on different types of substrates, including cheap and renewable sources like sunflower oil from deep fryers and sunflower oil mill effluent. Rhamnolipids were monitored by high-performance liquid chromatography–electrospray ionization interface mass spectrometry, which allowed fast and reliable identification and quantification of the congeners present. The highest concentration of total rhamnolipids of 3.33 g/l was obtained by the strain P. aeruginosa 67, recovered from petroleum contaminated soil, and strains D1 (1.73 g/l) and D2 (1.70 g/l), recovered from natural microbial consortia originated from mazut-contaminated soil, grown on sunflower oil as a carbon source. Di- to mono-rhamnolipids ratios were in the range of 0.90–5.39 for different media composition and from 1.12 to 4.17 for different producing strains. Rhamnolipid profiles of purified mixtures of all tested strains are similar with chain length from C8–C12, pronounced abundance of Rha–C10–C10 and Rha–Rha–C10–C10 congeners, and a low content of 3-(3-hydroxyalkanoyloxy)-alkanoic acids. Concentrations of major congeners of RLs were found to slightly vary, depending on strain and growth conditions, while variations in minor congeners were more pronounced. Statistically significant increase of critical micelle concentration values was observed with lowering the ratio of total mono- to di-rhamnolipids ratio indicating that mono-rhamnolipids start to form micelles at lower concentration than di-rhamnolipids. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-1d3c9c3e31783f58eb550761173bdca5-ff-Table-1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-7e8b85d996e5fb1eebaf3a614d01a77d-ff-Table-2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-0d37fed8633ba34e9f474844426e1cc6-ff-Table-3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=25919&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=25919" alt="SCImago Journal & Country Rank" /></a> | ||
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Rhamnolipids: diversity of structures, microbial origins and roles
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Review Article | 2010-03 | 10.1007/s00253-010-2498-2 | Applied Microbiology and Biotechnology | 86 | 1323–1336 | Citation file | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | Rhamnolipids are glycolipidic biosurfactants produced by various bacterial species. They were initially found as exoproducts of the opportunistic pathogen Pseudomonas aeruginosa and described as a mixture of four congeners: α-L-rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-Rha-C10-C10), α-L-rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoate (Rha-Rha-C10), as well as their mono-rhamnolipid congeners Rha-C10-C10 and Rha-C10. The development of more sensitive analytical techniques has lead to the further discovery of a wide diversity of rhamnolipid congeners and homologues (about 60) that are produced at different concentrations by various Pseudomonas species and by bacteria belonging to other families, classes, or even phyla. For example, various Burkholderia species have been shown to produce rhamnolipids that have longer alkyl chains than those produced by P. aeruginosa. In P. aeruginosa, three genes, carried on two distinct operons, code for the enzymes responsible for the final steps of rhamnolipid synthesis: one operon carries the rhlAB genes and the other rhlC. Genes highly similar to rhlA, rhlB, and rhlC have also been found in various Burkholderia species but grouped within one putative operon, and they have been shown to be required for rhamnolipid production as well. The exact physiological function of these secondary metabolites is still unclear. Most identified activities are derived from the surface activity, wetting ability, detergency, and other amphipathic-related properties of these molecules. Indeed, rhamnolipids promote the uptake and biodegradation of poorly soluble substrates, act as immune modulators and virulence factors, have antimicrobial activities, and are involved in surface motility and in bacterial biofilm development. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-7ed9f5ad32275b10e6606b608c311800-ff-Figure-1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-91e723be962690d6a24fa4b4f289bf19-ff-Table.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-11c978ab651e0a98df322c3d41bf0f47-ff-Table-2.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=14957&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=14957" alt="SCImago Journal & Country Rank" /></a> | ||
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Characterization of Rhamnolipid Produced by Pseudomonas aeruginosa Isolate Bs20
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Original Research Article | 2008-06 | 10.1007/s12010-008-8285-1 | Applied Biochemistry and Biotechnology | 157 | 329-345 | Citation file | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | Rhamnolipid produced by Pseudomonas aeruginosa isolate Bs20 is viscous sticky oily yellowish brown liquid with a fruity odor. It showed solubility at aqueous pH > 4 with optimum solubility at pH 7–7.5 and freely soluble in ethyl acetate. This biosurfactant has a very high surface activity as it could lower the surface tension of water to 30 mN/m at about 13.4 mg/L, and it exhibited excellent stabilities at high temperatures (heating at 100°C for 1 h and autoclaving at 121°C for 10 min), salinities (up to 6% NaCl), and pH values (up to pH 13). The produced biosurfactant can be used in the crude form either as cell-free or cell-containing culture broth of the grown bacteria, since both preparations showed high emulsification indices ranged between 59% and 66% against kerosene, diesel, and motor oil. These characters make the test rhamnolipid a potential candidate for use in bioremediation of hydrocarbon-contaminated sites or in the petroleum industry. High-performance thin-layer chromatography densitometry revealed that the extracted rhamnolipid contained the two most active rhamnolipid homologues dirhamno dilipidic rhamnolipid and monorhamno dilipidic rhamnolipid at 44% and 56%, respectively, as compared to 51% and 29.5%, respectively, in a standard rhamnolipid preparation. The nature and ratio of these two rhamnolipid homologues showed to be strain dependent rather than medium-component dependent. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-1085b795995884aabab5642e58736c1d-ff-Figure1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-75c303b9bbfa95fc6f046208653d1096-ff-Figure-2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-1dbea27c9ef163d1062f7ff438888f33-ff-ahmad-rl.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=110291&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=110291" alt="SCImago Journal & Country Rank" /></a> | ||
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Characterization of Surfactin Produced by Bacillus subtilis Isolate BS5
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Original Research Article | 2008-04 | 10.1007/s12010-008-8153-z | Applied Biochemistry and Biotechnology | 150 | 3 | 289-303 | Citation file | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | Physical and chromatographic characterization of the surfactin biosurfactant produced by Bacillus subtilis isolate BS5 has been conducted to study its potentiality for industrial application. The crude extract of test surfactin appeared as off-white to buff flake-like amorphous residue with bad odor similar to sour pomegranate. Test surfactin showed solubility in aqueous solution at pH > 5 with optimum solubility at pH 8–8.5. It was also soluble in organic solvents like ethanol, acetone, methanol, butanol, chloroform, and dichloromethane. Surfactin crystals appeared rectangular with blunt corners and were arranged perpendicular to each other making a plus sign. Extracted surfactin showed high surface activity, as it could lower the surface tension of water from about 70 to 36 mN/m at ~15.6 mg/l. Moreover, test surfactin exhibited excellent stabilities at high temperatures (100°C for up to 1 h at and autoclaving at 121°C for 10 min), salinities (up to 6% NaCl), and over a wide range of pH (5–13). Test surfactin in the cell-free supernatant or crude culture broth forms showed high emulsification indices against kerosene (62.5% and 59%, respectively), diesel (62.5% and 66%, respectively), and motor oil (62% and 66%, respectively). These characters can effectively make test surfactin, in its crude forms, a potential candidate for the use in bioremediation of hydrocarbon-contaminated sites or in the petroleum industry. Chromatographic characterization of test surfactin, using high-performance liquid chromatography technique, revealed that the extracted surfactin contained numerous isoforms, of which six were found in the standard surfactin preparation (Fluka). Additional peaks appeared in the test surfactin and not in the standard one. These peaks may correspond to new surfactin isoforms that may be present in the test surfactin produced by B. subtilis isolate BS5. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-6af8397aca0ef275bc1a4c61d88c08b6-ff-Figure1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-fc091a80f70cc006810a5a3541d5b5c1-ff-Figure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-fbf8c0ceab8d3d66f5f32b990120fc2e-ff-Figure3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=110291&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=110291" alt="SCImago Journal & Country Rank" /></a> | |
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Optimization of Surfactin Production by Bacillus subtilis Isolate BS5
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Original Research Article | 2008-02 | 10.1007/s12010-008-8155-x | Applied Biochemistry and Biotechnology | 150 | 305-325 | Citation file | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | Bacillus subtilis BS5 is a soil isolate that produces promising yield of surfactin biosurfactant in mineral salts medium (MSM). It was found that cellular growth and surfactin production in MSM were greatly affected by the environmental fermentation conditions and the medium components (carbon and nitrogen sources and minerals). Optimum environmental conditions for high surfactin production on the shake flask level were found to be a slightly acidic initial pH (6.5–6.8), an incubation temperature of 30°C, a 90% volumetric aeration percentage, and an inoculum size of 2% v/v. For media components, it was found that the optimum carbon source was molasses (160 ml/l), whereas the optimum nitrogen source was NaNO3 (5 g/l) and the optimum trace elements were ZnSO4·7H2O (0.16 g/l), FeCl3·6H2O (0.27 g/l), and MnSO4·H2O (0.017 g/l). A modified MSM (molasses MSM), combining the optimum medium components, was formulated and resulted in threefold increase in surfactin productivity that reached 1.12 g/l. No plasmid could be detected in the tested isolate, revealing that biosurfactant production by B. subtilis isolate BS5 is chromosomally mediated but not plasmid-mediated. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-42806ff49d7aad23e519b0c894193c17-ff-Figure1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-0ec7d075fb3468f98bd8a84eed65ad10-ff-Figure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-2161e9b431a4a0777325b598898b430e-ff-Figure3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=110291&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=110291" alt="SCImago Journal & Country Rank" /></a> | ||
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Microbial production of surfactants: Screening and identification of two promising isolates and their biosurfactants
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Original Research Article | 2007-10 | Egyptian Journal of Biotechnology | 27 | 166-185 | Citation file | A. M. Abdel-Mawgoud; M. M. Aboulwafa; N. A.-H. Hassouna | Microbial production of surfactants was tested in 1945 bacterial isolates. The tested isolates were recovered from 30 soil samples either contaminated with oil  products, oil products and iron or uncontaminated. Biosurfactant production was tested using 3-phases screening protocol. Primary screening was conducted by measurement of surface tension using the qualitative drop-collapse test (DCT) which resulted in the discovery of 28 high biosurfactant producing isolates. These isolates were subjected to secondary screening using a semi-quantitative microassay method for surfactants (Oil spreading test, OST) which resulted in the selection of 16 isolates (out of 28 high biosurfactant producers) that recorded highest scores. Tertiary screening was carried out on the 16 isolates using a du Nouy ring tensiometer for more sensitive quantitative measurement of surface tension. Of the 16 isolates tested in tertiary screening; a Gram positive isolate BS5, identified as Bacillus subtilis, and a Gram negative isolate BS20, identified as Pseudomonas aeruginosa, each showed the highest biosurfactant productivity compared to other members of its Gram group. The biosurfactant produced by B. subtilis isolate BS5 in mineral salts medium (MSM) was identified as surfactin, while, that produced by P. aeruginosa isolate BS20 was identified as rhamnolipid. TLC analysis revealed that surfactin showed one separated spot with an Rf value of 0.8, while, rhamnolipid biosurfactant showed two separated spots having Rf values of 0.4 and 0.68. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-f886df9501cb4b7dd6402b592ae6c882-ff-Figure1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-5d2b8f1ddfedf183a19820e5080464b3-ff-Figure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-9df317f9ccb80d6f69867d3d6659d3cb-ff-Figure3.jpg | ||||
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Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids
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Book Chapter | 2014-03 | 10.1007/978-1-4939-0473-0_30 | Pseudomonas Methods and Protocols. Methods in Molecular Biology | 1149 | 359–373 | Citation file | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | Rhamnolipids (RL) are surface-active glycolipids produced by Pseudomonas aeruginosa. They are always produced by this bacterium as a complex mixture of congeners, each composed of one or two rhamnose molecules linked to a dimer of 3-hydroxyfatty acids with a chain length of 8–12 carbons. Increasing interest for RL drives the need for efficient analytical methods to characterize these mixtures of molecules.High-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) is a very precise and relatively high-throughput method for the identification of each congener and their quantification in bacterial cultures. Using 13C-labeled RL as internal standards can further enhance the precision of the quantification. Collision-induced dissociation (CID) experiments by MS/MS is a powerful tool for the detection and identification of structural variations in RL produced by various Pseudomonas strains or by a specific strain under different culture conditions. CID even allows the discrimination between isomers with subtle structural variations, like Rha-C8-C10 and Rha-C10-C8, which are almost inseparable chromatographically. We are presenting here the detailed protocols for HPLC/MS and HPLC/MS/MS analysis of RL and their lipid precursors, the 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAA), directly in bacterial culture supernatants. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-509fa53077b55ee2024398d08ecc3a02-ff-RL-structures.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-280c72745a8b2d9b3ebcbc624fc81058-ff-Figure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-992e12512ce9b4ba7453d334c7568db2-ff-Figure3.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=12196&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=12196" alt="SCImago Journal & Country Rank" /></a> | ||
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Rhamnolipids: Detection, Analysis, Biosynthesis, Genetic Regulation, and Bioengineering of Production
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Book Chapter | 2010-09 | 10.1007/978-3-642-14490-5_2 | Biosurfactants. Microbiology Monographs | 20 | 13–55 | Citation file | A. M. Abdel-Mawgoud; R. Hausmann; F. Lépine; M. M. Müller; E. Déziel | As promising biotechnological products, rhamnolipids (RLs) are the most investigated biosurfactants. Over the years, important efforts have been spent and an array of techniques has been developed for the isolation of producing bacterial strains and the characterization of a large variety of RL homologs and congeners. Investigations on RL production by the best known producer, the opportunistic pathogen Pseudomonas aeruginosa, have shown that production of RLs proceeds through de novo biosynthesis of precursors. Over the last 15 years, the genetic details underlying RL production in P. aeruginosa have been mostly unraveled, revealing a complex regulatory mechanism controlled by quorum sensing pathways of intercellular communication. A number of nutritional and cultivation factors affecting RL productivity have also been identified, while the use of many affordable and renewable raw substrates has been described to optimize the production. Multidisciplinary approaches are increasingly adopted to develop methods for the safe, cost-effective, and highly efficient production of RLs at the industrial scale. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-636a4718c110dd50c287c48c87f7be15-ff-Figure1.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-4e86a90aaddca6dc6ba4dc191017f71b-ff-Figure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-9bcddc864249e3106a859d787af043e8-ff-Figure3.jpg | |||
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<a href="https://patents.google.com/patent/WO2015085411A1/en" target="_blank">Modulation of the beta-oxidation pathway in the control of rhamnolipid production</a>
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Patent | 2015-06 | World Intellectual Property Organization - PCT | 1-83 | A. M. Abdel-Mawgoud; F. Lépine; E. Déziel | Novel methods and agents for modulating the production of rhamnolipids (RLs) by a microorganism, through the modulation of the expression and/or activity of R-specific enoyl-CoA hydratases/isomerases (ECH/I), are described. Assays to identify agents that may be useful for modulating the production of RLs by a microorganism are also described. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-d1b59161795e1f49cd4894e6c5fbe29b-ff-Figure2.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-5576dd4f4b3bc8d358f6504449a77f36-ff-Figure3.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-2c6977f4e18dec8d834bf251ad2f6f04-ff-Figure4.jpg | ||||||
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Production of genetically engineeered designer biodiesel from yeast lipids
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Original Research Article | 2024-02 | 10.1016/j.crbiot.2024.100189 | Current Research in Biotechnology | 7 | 100189 | Citation file | B. Ouellet; A. M. Abdel-Mawgoud | Biodiesels constitute a growing class of fuel in the world which is increasingly inclined towards more ecological and sustainable energy. Despite their advantages, biodiesels are of limited cold flow properties and of larger NOx emissions, which are mostly attributed to the chemical composition of their oil feedstocks. This study presents a novel approach to produce Genetically Engineered Biodiesel (GEB) from genetically manipulated oleaginous yeast oils to be used for the production of biodiesels with improved properties and performances. Using full-factorial central composite design, the best chemical composition of an optimal biodiesel was predicted. Then, simple and combined MFE1, PEX10 and POX2 mutants of the oleaginous yeast Yarrowia lipolytica were constructed and showed interesting lipid profiles whose biodiesel is predicted to have better cold flow properties. These mutants showed also higher lipid titers by 2-3 folds compared to the parent strain. This study provides a genetic engineering strategy for tailor design of biodiesel properties and performance. Moreover, it provides solutions enabling biodiesel to be potentially used as a standalone fuel without any mixing with petrodiesel. | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-7baf625e5f1da32a85723f397f58a2e2-ff-Graphical-abstract.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-a66dc0d76e6b4296d3902d5067a41509-ff-CCD.jpg | https://abdel-mawgoud.com/wp-content/uploads/fluentform/ff-aad2ce23e75ffca4f3d8f245df9d3375-ff-Lipid-profiles.jpg | <a href="https://www.scimagojr.com/journalsearch.php?q=21101039902&tip=sid&exact=no" title="SCImago Journal & Country Rank"><img border="0" src="https://www.scimagojr.com/journal_img.php?id=21101039902" alt="SCImago Journal & Country Rank" /></a> |