We would like to inform you that the monthly online meeting of Synthetic Biology Club (SB club) will be held Today Monday, October 31 at 12h00 (EST). Please, Click here to register (if you did not do that yet) after which you will receive the meeting link (zoom).

Title: “Medium optimization for high lipid production by Yarrowia lipolytica and Rhodotorula toruloides using an optimized Nile Red-based lipid fluorometry”

Presenter: Zacharie Morneau, MSc student at the Department of biochemistry, microbiology and bioinformatics, Laval University, Canada and the Institute of Integrative and Systems Biology, Laval University, Canada.

Time: Monday, October 31, 2022 at 12h00 (EST).

Place: Online (via Zoom). The zoom link of the meeting is provided by email after your registration. Click here to register.

Abstract: As lipogenic yeasts are becoming increasingly harnessed as biofactories of oleochemicals, the availability of efficient protocols for the determination and optimization of lipid titers in these organisms is necessary. In this study, we optimized a quick, reliable and high throughput Nile Red-based lipid fluorometry protocol adapted for oleaginous yeasts and validated it using different approaches, the most important of which is using Gas chromatography coupled to flame ionization detection (GC-FID). This protocol was applied for the optimization the concentrations of ammonium chloride and glycerol for the highest lipid titers in Rhodotorula toruloides NRRL Y-6987 and Yarrowia lipolytica W29 using response surface central composite design (CCD). The optimal concentration of ammonium chloride and glycerol are 8,8 and 125 g/L, respectively, that achieved a C/N ratio ~25 above which lipid production decreases rapidly. The developed regression models and response surface plots give an insight on the careful selection of optimal C/N ratio for maximal lipid production